P-124: Protective Effect of Capper Hydroalcoholic Extract on Monusodium Glutamate-Induced damages on The Ovary of Rats; Evidence for Hsp70-2, p53 Genes Expressionn

Background: There has been a growing claim that cell phone usage may have a detrimental effect on sperm chromatin. Infertile men possess anomalies in the composition of their sperm nuclei, displaying higher levels of loosely packaged chromatin and damaged DNA. In this study we examined the relationship of cell phone radiation and the protection effect of alpha_tocopherol on human sperm DNA. Materials and Methods: Semen samples were collected from 12 patients presenting to the infertility clinic. Each samples was divided in two aliquots: control group(sample not exposed)and exposure group (sample exposed to cell phone).Exposure group was divided in two parts, the first was exposed to the cell phone radiation directly, the second group was mixed with 5mM alpha_tocophrol and emitted to cell phone radiation. Smears from each group were fixed in Carnoy’s fixative (methanol: acetic acid, 3:1) overnight. The slides were stained for 15 minutes and gently rinsed with PBS. The green and red fluorescence sperms were observed by fluorescence microscope. Results: The AO showed that the exposure group samples had a greater DNA damage if compared to control group. Furthermore adding alpha _tocophrol in semen during mobile phone exposure showed protection effect compare control and exposed group only significantly. Conclusion: Genotoxic effect of RF_EMW on sperms is either through ROS production or through direct clastogenic chromatin breaking effect. The addition of alpha_tocopherol in the semen could be useful to prevent human sperm chromatin after mobile phone radiation.